Difference between revisions of "Part:BBa K875002:Experience"
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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | ||
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===Applications of BBa_K875002=== | ===Applications of BBa_K875002=== | ||
| + | Trieste Team 2012 used this inducible promotor in the first phase of their project to test every construct that could have been toxic for the host strain, if expressed in a constitutive way. | ||
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| + | How it works: | ||
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| + | The host strain contains the low copy plasmid pREP4 which constitutively expresses the Lac repressor protein encoded by the lacI gene. The lac repressor binds the operator sequence preventing the transcription. The Lac repressor protein is rapidly inactivated by the binding of isopropyl-β-thiogalactoside (IPTG) | ||
===User Reviews=== | ===User Reviews=== | ||
Revision as of 17:20, 25 September 2012
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K875002
Trieste Team 2012 used this inducible promotor in the first phase of their project to test every construct that could have been toxic for the host strain, if expressed in a constitutive way.
How it works:
The host strain contains the low copy plasmid pREP4 which constitutively expresses the Lac repressor protein encoded by the lacI gene. The lac repressor binds the operator sequence preventing the transcription. The Lac repressor protein is rapidly inactivated by the binding of isopropyl-β-thiogalactoside (IPTG)
User Reviews
UNIQc4e5d2fac636781d-partinfo-00000000-QINU UNIQc4e5d2fac636781d-partinfo-00000001-QINU