Difference between revisions of "Part:BBa K917005:Experience"
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===Applications of BBa_K917005=== | ===Applications of BBa_K917005=== | ||
Expression of this biobrick inhibits growth of cells in the presence of nitro drugs such as metronidazole. We have rigorously tested this biobrick's potential as a counter-selectable marker in its pre-biobrick form (in Blue script vector with lac promoter).Although growth of cells containing nitroreductase is inhibited in plates, it is not absolute and therefore unsuitable for couter-selection. | Expression of this biobrick inhibits growth of cells in the presence of nitro drugs such as metronidazole. We have rigorously tested this biobrick's potential as a counter-selectable marker in its pre-biobrick form (in Blue script vector with lac promoter).Although growth of cells containing nitroreductase is inhibited in plates, it is not absolute and therefore unsuitable for couter-selection. | ||
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| + | https://static.igem.org/mediawiki/parts/8/85/Nitroreductase_plates.jpg | ||
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| + | Nitroreductase containing cells's growth (right) is markedly inhibited in comparison to control cells at 90 ug/ml metronidazole. However, this inhibition is not absolute so cannot be used for counterselection. | ||
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Aerobic liquid culture results are more promising with profound growth inhibition of nitroteductase-containing cells while control cells are growing. | Aerobic liquid culture results are more promising with profound growth inhibition of nitroteductase-containing cells while control cells are growing. | ||
Revision as of 16:10, 25 September 2012
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Applications of BBa_K917005
Expression of this biobrick inhibits growth of cells in the presence of nitro drugs such as metronidazole. We have rigorously tested this biobrick's potential as a counter-selectable marker in its pre-biobrick form (in Blue script vector with lac promoter).Although growth of cells containing nitroreductase is inhibited in plates, it is not absolute and therefore unsuitable for couter-selection.
Nitroreductase containing cells's growth (right) is markedly inhibited in comparison to control cells at 90 ug/ml metronidazole. However, this inhibition is not absolute so cannot be used for counterselection.
Aerobic liquid culture results are more promising with profound growth inhibition of nitroteductase-containing cells while control cells are growing.
Liquid aerobic cultures of BS-nitroreductase and BS-control in the presence and absence of metronidazole. At 150 ug/ml metronidazole, cells which contain nitroreductase show almost no growth. Error bars are standard error of the mean. Inital OD600 (just after inoculation) was subtracted.
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