Difference between revisions of "Part:BBa K917005:Experience"
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===Applications of BBa_K917005=== | ===Applications of BBa_K917005=== | ||
Expression of this biobrick inhibits growth of cells in the presence of nitro drugs such as metronidazole. We have rigorously tested this biobrick's potential as a counter-selectable marker in its pre-biobrick form (in Blue script vector with lac promoter).Although growth of cells containing nitroreductase is inhibited in plates, it is not absolute and therefore unsuitable for couter-selection. | Expression of this biobrick inhibits growth of cells in the presence of nitro drugs such as metronidazole. We have rigorously tested this biobrick's potential as a counter-selectable marker in its pre-biobrick form (in Blue script vector with lac promoter).Although growth of cells containing nitroreductase is inhibited in plates, it is not absolute and therefore unsuitable for couter-selection. | ||
| − | Aerobic liquid culture results are more promising with profound growth inhibition of nitroteductase-containing cells while control cells are growing. https://static.igem.org/mediawiki/parts/0/06/Nitroreductase_aerobic_culture.jpg | + | Aerobic liquid culture results are more promising with profound growth inhibition of nitroteductase-containing cells while control cells are growing. |
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| + | https://static.igem.org/mediawiki/parts/0/06/Nitroreductase_aerobic_culture.jpg | ||
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| + | Liquid aerobic cultures of BS-nitroreductase and BS-control in the presence and absence of metronidazole. At 150 ug/ml metronidazole, cells which contain nitroreductase show almost no growth. Error bars are standard error of the mean. Inital OD600 (just after inoculation) was subtracted. | ||
===User Reviews=== | ===User Reviews=== | ||
Revision as of 16:08, 25 September 2012
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Applications of BBa_K917005
Expression of this biobrick inhibits growth of cells in the presence of nitro drugs such as metronidazole. We have rigorously tested this biobrick's potential as a counter-selectable marker in its pre-biobrick form (in Blue script vector with lac promoter).Although growth of cells containing nitroreductase is inhibited in plates, it is not absolute and therefore unsuitable for couter-selection. Aerobic liquid culture results are more promising with profound growth inhibition of nitroteductase-containing cells while control cells are growing.
Liquid aerobic cultures of BS-nitroreductase and BS-control in the presence and absence of metronidazole. At 150 ug/ml metronidazole, cells which contain nitroreductase show almost no growth. Error bars are standard error of the mean. Inital OD600 (just after inoculation) was subtracted.
User Reviews
UNIQda6bce858cc2c54a-partinfo-00000000-QINU UNIQda6bce858cc2c54a-partinfo-00000001-QINU