Difference between revisions of "Part:BBa K782069"

Revision as of 16:04, 25 September 2012

10x[TALA+TALB] operator

Introduction

TAL effectors (TALEs) are able to specifically bind to the DNA by recognizing base pairs on a DNA molecule. With TAL regulators we are able to control expression of a desired gene. Efficient transcription control can be achieved by binding of multiple TAL regulators upstream of promoter.

Construct contain ten binding sites while single binding site combines 18 base pairs specificly recognised by TALA.

Single binding site sequence for TALA: TTTACTGCTGCTCCCGCT Binding sites are constructed in alternating setup. Besides TALA binding sites construct also contain ten alternating TALC binding sites, which we didn't use, since TALC wasn't working properly.

Figure 1: Schematic representation of the construct.

Characterization

Construct was later used for constructing different reporter plasmids to show specific binding of TALA KRAB / VP16. Results:

Sequence and Features