Difference between revisions of "Part:BBa K917005:Experience"
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===Applications of BBa_K917005=== | ===Applications of BBa_K917005=== | ||
| − | Expression of this biobrick inhibits growth of cells in the presence of nitro drugs such as metronidazole. We have rigorously tested this biobrick's potential as a counter-selectable marker in its pre-biobrick form (in Blue script vector with lac promoter).Although growth of cells containing nitroreductase is inhibited in plates, it is not absolute and therefore unsuitable for couter-selection. Aerobic liquid culture results are more promising with profound growth inhibition of nitroteductase-containing cells while control cells are growing. | + | Expression of this biobrick inhibits growth of cells in the presence of nitro drugs such as metronidazole. We have rigorously tested this biobrick's potential as a counter-selectable marker in its pre-biobrick form (in Blue script vector with lac promoter).Although growth of cells containing nitroreductase is inhibited in plates, it is not absolute and therefore unsuitable for couter-selection. |
| + | Aerobic liquid culture results are more promising with profound growth inhibition of nitroteductase-containing cells while control cells are growing. https://static.igem.org/mediawiki/parts/0/06/Nitroreductase_aerobic_culture.jpg | ||
===User Reviews=== | ===User Reviews=== | ||
Revision as of 16:02, 25 September 2012
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Applications of BBa_K917005
Expression of this biobrick inhibits growth of cells in the presence of nitro drugs such as metronidazole. We have rigorously tested this biobrick's potential as a counter-selectable marker in its pre-biobrick form (in Blue script vector with lac promoter).Although growth of cells containing nitroreductase is inhibited in plates, it is not absolute and therefore unsuitable for couter-selection.
Aerobic liquid culture results are more promising with profound growth inhibition of nitroteductase-containing cells while control cells are growing. 
User Reviews
UNIQ999ae21bb8a64358-partinfo-00000000-QINU UNIQ999ae21bb8a64358-partinfo-00000001-QINU