Difference between revisions of "Part:BBa K907000"
| Line 3: | Line 3: | ||
---- | ---- | ||
| − | + | <br> | |
[[Image: KAIST_DNA_recombination_via_Bxb1_Int,_Xis.png |center|450px]] | [[Image: KAIST_DNA_recombination_via_Bxb1_Int,_Xis.png |center|450px]] | ||
| − | This part is a protein coding | + | This part is a protein coding part which encodes '''DNA integrase''' of ''Mycobacterium phage Bxb1''. |
The Bxb1 integrase is a DNA recombinase, more precisely a member of serine integrase family. It recognizes specific sequences, called ''attB'' and ''attP'', and then integrates, inverts, or excises dsDNA depending on the orientation of recognition sequences. | The Bxb1 integrase is a DNA recombinase, more precisely a member of serine integrase family. It recognizes specific sequences, called ''attB'' and ''attP'', and then integrates, inverts, or excises dsDNA depending on the orientation of recognition sequences. | ||
We used this integrase to invert specific sequence contained in plasmid. This protein is well expressed in ''E.coli(strain MG1655)''. | We used this integrase to invert specific sequence contained in plasmid. This protein is well expressed in ''E.coli(strain MG1655)''. | ||
Revision as of 15:40, 25 September 2012
Mycobacterium Phage Bxb1 gp35, DNA integrase
This part is a protein coding part which encodes DNA integrase of Mycobacterium phage Bxb1.
The Bxb1 integrase is a DNA recombinase, more precisely a member of serine integrase family. It recognizes specific sequences, called attB and attP, and then integrates, inverts, or excises dsDNA depending on the orientation of recognition sequences. We used this integrase to invert specific sequence contained in plasmid. This protein is well expressed in E.coli(strain MG1655). When it inverts DNA, the attB and attP sequences are changed into attL and attR, as other DNA recombinases do. Another protein called Bxb1 excisionase forms complex with integrase and this complex flips inverted DNA back into original sequence by regenerating attB and attP sequences.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 192
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 466
Illegal XhoI site found at 553 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1105
Illegal NgoMIV site found at 1192
Illegal AgeI site found at 242 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1300