Difference between revisions of "Part:BBa K738002"
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<partinfo>BBa_K738002 short</partinfo> | <partinfo>BBa_K738002 short</partinfo> | ||
| − | Scaffold D0 was constructed from a single RNA module d0, which folded into a duplex with PP7 and MS2 aptamer domains that bind PP7 and MS2 fusion proteins. A | + | Scaffold D0 was constructed from a single RNA module d0, which folded into a duplex with PP7 and MS2 aptamer domains that bind PP7 and MS2 fusion proteins. A designed theophylline aptamer was added on the original scaffold D0([BBa_K738002]) in order to produce an interaction with MS2 aptamer in the absence of theophylline, thus disturbing the bind of MS2 aptamer and corresponding protein. |
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===Usage and Biology=== | ===Usage and Biology=== | ||
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
<partinfo>BBa_K738002 SequenceAndFeatures</partinfo> | <partinfo>BBa_K738002 SequenceAndFeatures</partinfo> | ||
Revision as of 15:31, 25 September 2012
Theophyline riboswitch regulated RNA Scaffold (clover vision 2)
Scaffold D0 was constructed from a single RNA module d0, which folded into a duplex with PP7 and MS2 aptamer domains that bind PP7 and MS2 fusion proteins. A designed theophylline aptamer was added on the original scaffold D0([BBa_K738002]) in order to produce an interaction with MS2 aptamer in the absence of theophylline, thus disturbing the bind of MS2 aptamer and corresponding protein.
Usage and Biology
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]