Difference between revisions of "Part:BBa K863005:Experience"

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University College London 2012

As part of our collaboration with Bielefeld-Germany, we have characterised their Laccase BioBrick utilising a Syringaldazine assay to determine their Laccase activity. Syringaldazine is oxidised by Laccase, causing a colourmetric change that can be observed by a spectrophotometer at 530nm. As seen in the graph below, the highest rate of Laccase production is in transformed BL21 cells - this is to be expected due to the T7 promoter, which requires T7 RNA polymerase that BL21 produces. However, expression in W3110, which does not produce T7 RNA polymerase, is still elevated above that of untransformed E. coli, though not at the level of expression of transformed BL21. We hence conclude that Bielefeld-Germany's Laccase BioBrick is working as expected.

UniversityCollegeLondon CollaborationLaccase.png

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 <I>University College London 2012</I>
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-As part of our collaboration with Bielefeld-Germany, we have characterised their Laccase BioBrick utilising a Syringaldazine assay to determine their Laccase activity. Syringaldazine is oxidised by Laccase, causing a colourmetric change that can be observed by a spectrophotometer at 530nm. As seen in the graph below, the highest rate of Laccase production is in transformed BL21 cells - this is to be expected due to the T7 promoter, which requires T7 RNA polymerase that BL21 produces. However, expression in W3110, which does not produce T7 RNA polymerase, is still elevated above that of untransformed ''E. coli''. We hence conclude that Bielefeld-Germany's Laccase BioBrick is working as expected.
+As part of our collaboration with Bielefeld-Germany, we have characterised their Laccase BioBrick utilising a Syringaldazine assay to determine their Laccase activity. Syringaldazine is oxidised by Laccase, causing a colourmetric change that can be observed by a spectrophotometer at 530nm. As seen in the graph below, the highest rate of Laccase production is in transformed BL21 cells - this is to be expected due to the T7 promoter, which requires T7 RNA polymerase that BL21 produces. However, expression in W3110, which does not produce T7 RNA polymerase, is still elevated above that of untransformed ''E. coli'', though not at the level of expression of transformed BL21. We hence conclude that Bielefeld-Germany's Laccase BioBrick is working as expected.
 [[Image:UniversityCollegeLondon_CollaborationLaccase.png|800px|centre]]
 |};