Difference between revisions of "Part:BBa K887002:Design"
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− | + | It's improve form NCTU-Formosa 2011 iGEM team. | |
===References=== | ===References=== |
Revision as of 14:19, 25 September 2012
Plac+B0034+alsS+B0034+ilvC+B0034+ilvD+37℃RBS+tetR+double terminator+Ptet+B0032+kivD+B0015
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 6860
Illegal XhoI site found at 5191 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 6080
Illegal AgeI site found at 2560 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 2146
Illegal BsaI site found at 4977
Illegal BsaI site found at 5234
Illegal BsaI.rc site found at 534
Illegal BsaI.rc site found at 1128
Illegal BsaI.rc site found at 2965
Design Notes
The first part of this complete biobrick is built in Plac, two strong and one weak expressing RBS, Alss, ilvC, ilvD, 37°C regulator RBS, tetR and double terminator. The second part of this complete biobrick is start with Ptet,one RBS,and end with terminatorPart:BBa_B0015 is included.
Source
It's improve form NCTU-Formosa 2011 iGEM team.