Difference between revisions of "Part:BBa K747002"
| Line 12: | Line 12: | ||
|- | |- | ||
|Backbone e. g. TAL-TF | |Backbone e. g. TAL-TF | ||
| − | | | + | |200 ng ??? |
|- | |- | ||
|BsmBI | |BsmBI | ||
| − | |1,5 | + | |1,5 µl |
|- | |- | ||
|T4 DNA Ligase (60 u) | |T4 DNA Ligase (60 u) | ||
| − | |1,0 | + | |1,0 µl |
|- | |- | ||
|T4 DNA Ligase Buffer | |T4 DNA Ligase Buffer | ||
| − | |2,0 | + | |2,0 µl |
|- | |- | ||
|Water, nuclease-free | |Water, nuclease-free | ||
| − | |up to | + | |up to 20 µl |
|- | |- | ||
|'''Total vloume''' | |'''Total vloume''' | ||
Revision as of 13:42, 25 September 2012
TAL-Protein_AG1_Direpeat
Pipette the following volumes in one tube:
| Component | Volume |
| 6 Direpeats of your choice | 63,3 ng |
| Backbone e. g. TAL-TF | 200 ng ??? |
| BsmBI | 1,5 µl |
| T4 DNA Ligase (60 u) | 1,0 µl |
| T4 DNA Ligase Buffer | 2,0 µl |
| Water, nuclease-free | up to 20 µl |
| Total vloume | 20 µl |
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]