Difference between revisions of "Part:BBa K747002"
Line 17: Line 17:
 
|1,5
 
|1,5
 
|-
 
|-
−
|T4 DNA Ligase
+
|T4 DNA Ligase (60 u)
 
|1,0
 
|1,0
 
|-
 
|-
 
|T4 DNA Ligase Buffer
 
|T4 DNA Ligase Buffer
 
|2,0
 
|2,0
 +
|-
 +
|Water, nuclease-free
 +
|up to 20µl
 +
|-
 +
|'''Total vloume'''
 +
|'''20 µl'''
 
|}
 
|}
 
<br/>
 
<br/>

Revision as of 13:41, 25 September 2012

TAL-Protein_AG1_Direpeat

Pipette the following volumes in one tube:

Component Volume
6 Direpeats of your choice 63,3 ng
Backbone e. g. TAL-TF 1,5
BsmBI 1,5
T4 DNA Ligase (60 u) 1,0
T4 DNA Ligase Buffer 2,0
Water, nuclease-free up to 20µl
Total vloume 20 µl




Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]