Difference between revisions of "Part:BBa K747002"
Line 8: | Line 8: | ||
|'''Volume''' | |'''Volume''' | ||
|- | |- | ||
− | |6 Direpeats | + | |6 Direpeats of your choice |
− | of your choice | + | |
|63,3 ng | |63,3 ng | ||
|- | |- | ||
− | |Backbone e. g. | + | |Backbone e. g. TAL-TF |
− | TAL-TF | + | |
|1,5 | |1,5 | ||
|- | |- |
Revision as of 13:36, 25 September 2012
TAL-Protein_AG1_Direpeat
Pipette the following volumes in one tube:
Component | Volume |
6 Direpeats of your choice | 63,3 ng |
Backbone e. g. TAL-TF | 1,5 |
BsmBI | 1,5 |
T4 DNA Ligase | 1,0 |
T4 DNA Ligase Buffer | 2,0 |
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]