Difference between revisions of "Part:BBa K747002"
| Line 12: | Line 12: | ||
|63,3 ng | |63,3 ng | ||
|- | |- | ||
| − | | | + | |Backbone e. g. |
| + | TAL-TF | ||
|1,5 | |1,5 | ||
|- | |- | ||
|BsmBI | |BsmBI | ||
|1,5 | |1,5 | ||
| + | |- | ||
| + | |T4 DNA Ligase | ||
| + | |1,0 | ||
| + | |- | ||
| + | |T4 DNA Ligase Buffer | ||
| + | |2,0 | ||
|} | |} | ||
<br/> | <br/> | ||
Revision as of 13:35, 25 September 2012
TAL-Protein_AG1_Direpeat
Pipette the following volumes in one tube:
| Component | Volume |
| 6 Direpeats
of your choice |
63,3 ng |
| Backbone e. g.
TAL-TF |
1,5 |
| BsmBI | 1,5 |
| T4 DNA Ligase | 1,0 |
| T4 DNA Ligase Buffer | 2,0 |
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]