Difference between revisions of "Part:BBa K784043"

Revision as of 13:25, 25 September 2012

Mutant T7 RNAP - T7*

This part includes an engineered T7 RNAP with a RBS, the RNAP recognizes specifically a suitable promoter (pT7). The mutant T7 RNAP was donated us by Chris Voight. The T7 RNAP part contains a weak ribosome binding site and 'GTG' start codon . The native T7 RNAP differs from the mutant one by its toxicity for the host cell- the mutant have a low toxicity than the native one (according to Voight's article), this treat for low toxicity was accrued by a Spontaneous mutation, within the active site, during the cloning and doesn’t reduce the RNAP activity. The part was made by cloning the T7 RNAP gene to the BioBrick BBa_B0015, which includes double terminator (B0010-B0012). This part is present in the plasmid pSB1AK3. The purpose of this part is that you can clone to it any gene you want to be combined with it: promoter, reporter gene etc..
Note: the biobrick prefix has been altered to deletion of one bp downstream to the XbaI site. This was due to wrong primers.

Usage and Biology

Sequence and Features