Difference between revisions of "Part:BBa K784043"
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The native T7 RNAP differs from the mutant one by its toxicity for the host cell- the mutant have a low toxicity than the native one (according to Voight's article), this treat for low toxicity was accrued by a Spontaneous mutation, within the active site, during the cloning and doesn’t reduce the RNAP activity. | The native T7 RNAP differs from the mutant one by its toxicity for the host cell- the mutant have a low toxicity than the native one (according to Voight's article), this treat for low toxicity was accrued by a Spontaneous mutation, within the active site, during the cloning and doesn’t reduce the RNAP activity. | ||
The part was made by cloning the T7 RNAP gene to the BioBrick BBa_B0015, which includes double terminator (B0010-B0012). This part is present in the plasmid pSB1AK3. | The part was made by cloning the T7 RNAP gene to the BioBrick BBa_B0015, which includes double terminator (B0010-B0012). This part is present in the plasmid pSB1AK3. | ||
| − | The purpose of this part is that you can clone to it any gene you want to be combined with it: promoter, reporter gene etc.. | + | The purpose of this part is that you can clone to it any gene you want to be combined with it: promoter, reporter gene etc..<br> |
| − | Note: the biobrick prefix has been altered to deletion of one bp downstream to the XbaI site. | + | Note: the biobrick prefix has been altered to deletion of one bp downstream to the XbaI site. This was due to wrong primers. |
===Usage and Biology=== | ===Usage and Biology=== | ||
Revision as of 13:01, 25 September 2012
T7*(K1F) RNA polymerase
This part includes an engineered T7 RNAP with a RBS, the RNAP recognizes specifically a suitable promoter (pT7). The mutant T7 RNAP was donated us by Chris Voight.
The T7 RNAP part contains a weak ribosome binding site and 'GTG' start codon .
The native T7 RNAP differs from the mutant one by its toxicity for the host cell- the mutant have a low toxicity than the native one (according to Voight's article), this treat for low toxicity was accrued by a Spontaneous mutation, within the active site, during the cloning and doesn’t reduce the RNAP activity.
The part was made by cloning the T7 RNAP gene to the BioBrick BBa_B0015, which includes double terminator (B0010-B0012). This part is present in the plasmid pSB1AK3.
The purpose of this part is that you can clone to it any gene you want to be combined with it: promoter, reporter gene etc..
Note: the biobrick prefix has been altered to deletion of one bp downstream to the XbaI site. This was due to wrong primers.
Usage and Biology
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]