Difference between revisions of "Part:BBa K823029"
Line 12: Line 12:
 
Emission maximum: 633 nm
 
Emission maximum: 633 nm
  
−
[[Image:MKate Pellet.JPG|<p align="justify">'''''mKate2'' fused to the terminator B0014 under the control of the Anderson promoter J23101 (up), P<sub>''liaI''</sub> (middle) and [https://parts.igem.org/Part:BBa_K823002 P<sub>''lepA''</sub>] (down) in [https://parts.igem.org/Part:BBa_K8230023 pSB<sub>''Bs''</sub>1C]'''. Pellets are ''Escherichia coli'' cells which contain the plasmid with the right insert.</p>|thumb|300px|left]]
+
[[Image:LMU-Munich-MKate Pellet.JPG|<p align="justify">'''''mKate2'' fused to the terminator B0014 under the control of the Anderson promoter J23101 (up), P<sub>''liaI''</sub> (middle) and [https://parts.igem.org/Part:BBa_K823002 P<sub>''lepA''</sub>] (down) in [https://parts.igem.org/Part:BBa_K8230023 pSB<sub>''Bs''</sub>1C]'''. Pellets are ''Escherichia coli'' cells which contain the plasmid with the right insert.</p>|thumb|300px|left]]
 
<p align="justify">We cloned this reporter in front of the terminator B0014. For the evaluation this reporter was successfully combined with the promoters [https://parts.igem.org/Part:BBa_K823001 P<sub>''liaI''</sub>], P<sub>''lepA''</sub> and the Anderson promoter J23101 in the empty ''Bacillus'' vector pSB<sub>''Bs''</sub>1C from our '''''Bacillus''B'''io'''B'''rick'''B'''ox. At the moment we have the right clones of ''B. subtilis'' with the integrated construct. Unfortunately we have no equipment to measure this reporter. Neither our plate reader nor the fluorescent microscope has the required filters.</p>
 
<p align="justify">We cloned this reporter in front of the terminator B0014. For the evaluation this reporter was successfully combined with the promoters [https://parts.igem.org/Part:BBa_K823001 P<sub>''liaI''</sub>], P<sub>''lepA''</sub> and the Anderson promoter J23101 in the empty ''Bacillus'' vector pSB<sub>''Bs''</sub>1C from our '''''Bacillus''B'''io'''B'''rick'''B'''ox. At the moment we have the right clones of ''B. subtilis'' with the integrated construct. Unfortunately we have no equipment to measure this reporter. Neither our plate reader nor the fluorescent microscope has the required filters.</p>
  

Revision as of 12:47, 25 September 2012

mKate2, a red monomeric fluorescent protein, B. subtilis optimized

mKate2 is a far-red fluorescent protein that is monomeric and extremely photostable. It is in Freiburg standard and has a RBS included with the correct spacing.

prefix: GAATTCCGCGGCCGCTTCTAGATAAGGAGGAACTACTATGGCCGGC

suffix: ACCGGTTAATACTAGTAGCGGCCGCTGCAGT

Excitation maximum: 588 nm

Emission maximum: 633 nm

mKate2 fused to the terminator B0014 under the control of the Anderson promoter J23101 (up), PliaI (middle) and PlepA (down) in pSBBs1C. Pellets are Escherichia coli cells which contain the plasmid with the right insert.

We cloned this reporter in front of the terminator B0014. For the evaluation this reporter was successfully combined with the promoters PliaI, PlepA and the Anderson promoter J23101 in the empty Bacillus vector pSBBs1C from our BacillusBioBrickBox. At the moment we have the right clones of B. subtilis with the integrated construct. Unfortunately we have no equipment to measure this reporter. Neither our plate reader nor the fluorescent microscope has the required filters.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 4
  • 1000
    COMPATIBLE WITH RFC[1000]