Difference between revisions of "Part:BBa K911007"
| Line 2: | Line 2: | ||
<partinfo>BBa_K911007 short</partinfo> | <partinfo>BBa_K911007 short</partinfo> | ||
| − | See part BBa_K911008 for a similar part. | + | See part [https://parts.igem.org/wiki/index.php?title=Part:BBa_K911008 BBa_K911008] for a similar part. |
This part upregulates an operon responsible for germination rate. Bacillus subtilis spores germinate in response L-Alanine. Up-regulation of the spoVA operon increases germination rate in response to L-Alanine. The promoter for the B.subtilis sspB gene (PsspB) is more active than the endogenous spoVA promoter. It is also active during germination. Expression of the spoVA operon under PsspB increases the germination rate. | This part upregulates an operon responsible for germination rate. Bacillus subtilis spores germinate in response L-Alanine. Up-regulation of the spoVA operon increases germination rate in response to L-Alanine. The promoter for the B.subtilis sspB gene (PsspB) is more active than the endogenous spoVA promoter. It is also active during germination. Expression of the spoVA operon under PsspB increases the germination rate. | ||
Revision as of 11:32, 25 September 2012
Fast Germination (B.subtilis)
See part BBa_K911008 for a similar part.
This part upregulates an operon responsible for germination rate. Bacillus subtilis spores germinate in response L-Alanine. Up-regulation of the spoVA operon increases germination rate in response to L-Alanine. The promoter for the B.subtilis sspB gene (PsspB) is more active than the endogenous spoVA promoter. It is also active during germination. Expression of the spoVA operon under PsspB increases the germination rate.
This construct consists mainly of the sspB promoter followed by the first 354bp of the spoVA operon (first 354bp of the spoVAA gene). Since B.subtilis exhibits accurate and efficient homologous recombination, a single cross-over event between the spoVAA region in the BioBrick and the endogenous spoVAA sequence inserts the sspB promoter upstream of the spoVA operon.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]