Difference between revisions of "Part:BBa K864200:Design"
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<partinfo>BBa_K864200 short</partinfo> | <partinfo>BBa_K864200 short</partinfo> | ||
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===Source=== | ===Source=== | ||
| − | Amplified from MG1655 (Accession NC_000913) chromosome using primers listed below | + | Amplified from MG1655 (Accession NC_000913) chromosome using primers listed below (Annealing sequences are <u>underlined</u>) |
marR_BB_F | marR_BB_F | ||
| − | + | TTCGAATTCGCGGCCGCTTCTAGA<u>TGAAAAGTACCAGCGATCT</u> | |
marR_BB_R | marR_BB_R | ||
| − | + | GGCCGCTACTAGTATTA<u>TTACGGCAGGACTTTCTT</u> | |
===References=== | ===References=== | ||
Revision as of 09:57, 25 September 2012
MarR native E. coli repressor
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
None
Source
Amplified from MG1655 (Accession NC_000913) chromosome using primers listed below (Annealing sequences are underlined)
marR_BB_F TTCGAATTCGCGGCCGCTTCTAGATGAAAAGTACCAGCGATCT
marR_BB_R GGCCGCTACTAGTATTATTACGGCAGGACTTTCTT