Difference between revisions of "Part:BBa K819005"

Revision as of 07:48, 25 September 2012

Constitutive LuxBrick Generator

a fusion protein consisting of E.coli endogenous SOS system repressor LexA and fungus N.crass photosensor protein VVD with LexA carrying mutation at position 40-42 and VVD carrying mutation N56K, C71V and M135I. This part serves as an ultra sensitive photoreceptor(which can sense light as weak as moonlight) and will induce a light-dependent repression of genes with their promoter containing mutated 408 form of SOS box.

Luminesensor is designed by following the general principle of optogenetics fusion protein design--attaching a physiologically functional domain to a photoreceptor domain.

FIG.1 The predicted structure based on the data of T7 polymerase

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 3014
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 2012
Illegal XhoI site found at 2842
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 4401
Illegal BsaI.rc site found at 1410
Illegal SapI.rc site found at 4726

@@ Line 6: / Line 6: @@
 ''Luminesensor'' is designed by following the general principle of optogenetics fusion protein design--attaching a physiologically functional domain to a photoreceptor domain.
-<img src="/wiki/images/5/55/Peking2012_Design_illustration_of_general_principle_1.png"
+<html><a href="https://static.igem.org/mediawiki/2012/5/55/Peking2012_Design_illustration_of_general_principle_1.png"target="blank"><img src="https://static.igem.org/mediawiki/2012/5/55/Peking2012_Design_illustration_of_general_principle_1.png" width=350  ></a></html>
+'''FIG.1 The predicted structure based on the data of T7 polymerase'''
 <!-- Add more about the biology of this part here