Difference between revisions of "Part:BBa K792004"

Revision as of 07:21, 25 September 2012

Import enhancer - Polyarginine (trojan peptide)

Trojan peptides are short sequences that penetrate through the plasma membrane inside the cell without the need of any receptor or endocitosis process [Derossi 1998]. They can be used to increase the efficiency with which a protein enters a cell.

This part is Polyarginine, and has to be used just before the coding sequence of the peptide that you want to be import enhanced.

This part includes a HindIII restriction site as an extra.

(Refer to Characterisation of cell-penetrating peptide-mediated peptide delivery [Jones et al 2005] for more information about polyarginine)

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K792004 short</partinfo>
-Trojan peptides are short sequences that penetrate through the plasma membrane inside the cell without the need of any receptor or endocitosis process [Derossi 1998]. They can be used to increase the efficiency with which a protein enters a cell.
-This part is Polyarginine, and has to be used just before the coding sequence of the peptide that you want to be import enhanced.
+Trojan peptides are short sequences that penetrate through the plasma membrane inside the cell without the need of any receptor or endocitosis process ''[Derossi 1998]''. They '''can be used to increase the efficiency with which a protein enters a cell'''.
-This part includes a ''HindIII'' restriction site as an extra.
-(refer to ''Characterisation of cell-penetrating peptide-mediated peptide delivery'' [Jones et al 2005] for more information about polyarginine)
+This part is Polyarginine, and '''has to be used just before the coding sequence of the peptide''' that you want to be import enhanced.
+This part includes a '''HindIII''' restriction site as an extra.
+(Refer to ''Characterisation of cell-penetrating peptide-mediated peptide delivery'' [Jones et al 2005] for more information about polyarginine)