Difference between revisions of "Part:BBa K792011"
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The high level structure of the device is: | The high level structure of the device is: | ||
| − | * '''''Kozak''''' consensus sequence for initiation of translation | + | * '''''Kozak''''' consensus sequence for initiation of translation |
| − | * '''''Signal''''' peptide that targets the product of the gene for secretion | + | * '''''Signal''''' peptide that targets the product of the gene for secretion |
| − | * '''''Trojan''''' peptide, to increase internalization in target cell ''( | + | * '''''Trojan''''' peptide, to increase internalization in target cell ''(Polyarginine)'' |
* '''''Payload''''': this is the exported '''Histidine''' rich domain of the protein ''(a custom designed peptide that we called PolyHb)'' | * '''''Payload''''': this is the exported '''Histidine''' rich domain of the protein ''(a custom designed peptide that we called PolyHb)'' | ||
Revision as of 07:10, 25 September 2012
Yeast exportable His-rich peptide w/enhanced import (2)
This composite device produces and exports a Histidine rich peptide, that is import enhanced (thanks to a trojan peptide). This device is intended to be used with Yeast chassis, so it is composed by subparts designed also to be use in yeast.
The high level structure of the device is:
- Kozak consensus sequence for initiation of translation
- Signal peptide that targets the product of the gene for secretion
- Trojan peptide, to increase internalization in target cell (Polyarginine)
- Payload: this is the exported Histidine rich domain of the protein (a custom designed peptide that we called PolyHb)
The device's sequence has inherited some useful restriction sites (BamHI & HindIII) from the parts it's made of.
Important note: this part has been submitted to the registry using a direct synthesis DNA sample, so it does not contain scars from BB assembly standard.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]