Difference between revisions of "Part:BBa K864502"
| Line 2: | Line 2: | ||
<partinfo>BBa_K864502 short</partinfo> | <partinfo>BBa_K864502 short</partinfo> | ||
| − | Similar to the common RFP cassette <partinfo>BBa_J04450</partinfo>, but driven by a constitutive promoter. Useful for LacI positive systems or in LacIq backbones as a default insert, especially together with a high copy origin as in <partinfo> | + | Similar to the common RFP cassette <partinfo>BBa_J04450</partinfo>, but driven by a constitutive promoter. Useful for LacI positive systems or in LacIq backbones as a default insert, especially together with a high copy origin as in <partinfo>BBa_K864121</partinfo>. |
When expressed in a high copy vector, the colonies are clearly red in color under natural light after about 18 hours. Smaller colonies are visibly red under UV. The RFP part does not contain a degradation tag and the RBS is strong. | When expressed in a high copy vector, the colonies are clearly red in color under natural light after about 18 hours. Smaller colonies are visibly red under UV. The RFP part does not contain a degradation tag and the RBS is strong. | ||
Latest revision as of 06:42, 25 September 2012
RFP Coding Device, constitutive
Similar to the common RFP cassette BBa_J04450, but driven by a constitutive promoter. Useful for LacI positive systems or in LacIq backbones as a default insert, especially together with a high copy origin as in BBa_K864121.
When expressed in a high copy vector, the colonies are clearly red in color under natural light after about 18 hours. Smaller colonies are visibly red under UV. The RFP part does not contain a degradation tag and the RBS is strong.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 640
Illegal AgeI site found at 752 - 1000COMPATIBLE WITH RFC[1000]