Difference between revisions of "Part:BBa K792010"
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<partinfo>BBa_K792010 short</partinfo> | <partinfo>BBa_K792010 short</partinfo> | ||
| − | This composite device produces and '''exports''' a ''' | + | This composite device produces and '''exports''' a '''Tryptophan rich''' peptide, that is '''import enhanced''' (thanks to a trojan peptide). |
This device is intended to be used with '''Yeast chassis''', so it is composed by subparts designed also to be use in yeast. | This device is intended to be used with '''Yeast chassis''', so it is composed by subparts designed also to be use in yeast. | ||
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* '''''Signal''''' peptide that targets the product of the gene for secretion ''(also from yeast α-factor mating pheromone (MFα1))'' | * '''''Signal''''' peptide that targets the product of the gene for secretion ''(also from yeast α-factor mating pheromone (MFα1))'' | ||
* '''''Trojan''''' peptide, to increase internalization in target cell ''(from HIV TAT penetratin)'' | * '''''Trojan''''' peptide, to increase internalization in target cell ''(from HIV TAT penetratin)'' | ||
| − | * '''''Payload''''': this is the exported ''' | + | * '''''Payload''''': this is the exported '''Tryptophan''' rich domain of the protein ''(a TrpZipper)'' |
Revision as of 06:32, 25 September 2012
Yeast exportable Trp-rich peptide w/enhanced import (1)
This composite device produces and exports a Tryptophan rich peptide, that is import enhanced (thanks to a trojan peptide). This device is intended to be used with Yeast chassis, so it is composed by subparts designed also to be use in yeast.
The high level structure of the device is:
- Kozak consensus sequence for initiation of translation (from yeast α-factor mating pheromone (MFα1))
- Signal peptide that targets the product of the gene for secretion (also from yeast α-factor mating pheromone (MFα1))
- Trojan peptide, to increase internalization in target cell (from HIV TAT penetratin)
- Payload: this is the exported Tryptophan rich domain of the protein (a TrpZipper)
The device's sequence has inherited some useful restriction sites (BamHI & HindIII) from the parts it's made of.
Important note: this part has been submitted to the registry using a direct synthesis DNA sample, so it does not contain scars from BB assembly standard.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]