Difference between revisions of "Part:BBa K864511"
| Line 3: | Line 3: | ||
This is a constitutive promoter characterized in ''E. coli'' and ''Lactococcus lactis''. It has a strength of 34 (in ''E coli'') and 12 (in ''L lactis'') Miller units in the β-Galactosidase assay of Jensen & Hammer (1998). That makes it into a quite strong promoter in ''E coli'' and a rather weak promoter in ''L lactis'' (note that the scales are cannot be directly compared). | This is a constitutive promoter characterized in ''E. coli'' and ''Lactococcus lactis''. It has a strength of 34 (in ''E coli'') and 12 (in ''L lactis'') Miller units in the β-Galactosidase assay of Jensen & Hammer (1998). That makes it into a quite strong promoter in ''E coli'' and a rather weak promoter in ''L lactis'' (note that the scales are cannot be directly compared). | ||
| + | |||
| + | ===Usage and Biology=== | ||
| + | The CP series of Jensen & Hammer are useful as constitutive promoters when the restriction sites included in the Anderson promoter collection are illegal. | ||
===References=== | ===References=== | ||
Revision as of 05:58, 25 September 2012
CP44 constitutive promoter
This is a constitutive promoter characterized in E. coli and Lactococcus lactis. It has a strength of 34 (in E coli) and 12 (in L lactis) Miller units in the β-Galactosidase assay of Jensen & Hammer (1998). That makes it into a quite strong promoter in E coli and a rather weak promoter in L lactis (note that the scales are cannot be directly compared).
Usage and Biology
The CP series of Jensen & Hammer are useful as constitutive promoters when the restriction sites included in the Anderson promoter collection are illegal.
References
[http://www.ncbi.nlm.nih.gov/pmc/articles/PMC124675/] P. Jensen, K Hammer Appl: The Sequence of Spacers between the Consensus Sequences Modulates the Strength of Prokaryotic Promoters Environ Microbiol. 64.1 (1998) 82–87.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]