Difference between revisions of "Part:BBa K819000:Design"
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===Source===
 
===Source===
−
[[Image:[Example.jpg]https://static.igem.org/mediawiki/2012/7/71/Peking2012_team_zhangzidong_formal.jpg]]
+
https://static.igem.org/mediawiki/2012/7/71/Peking2012_team_zhangzidong_formal.jpg
 
ColE7 promoter, ColE plasmid
 
ColE7 promoter, ColE plasmid
  
 
===References===
 
===References===

Revision as of 04:51, 25 September 2012

Luminescence-responsive Promoter+GFP(BBa_E0840)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The LexA binding site was mutated to guarantee that the endogenous LexA will not disturb the function of this promoter. This mutated LexA binding site within luminescence-responsive promoter can be recognized by corresponding mutated LexA DNA binding domain.


Source

Peking2012_team_zhangzidong_formal.jpg ColE7 promoter, ColE plasmid

References