Difference between revisions of "Part:BBa K792004"
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This part is Polyarginine, and has to be used just before the coding sequence of the peptide that you want to be import enhanced. | This part is Polyarginine, and has to be used just before the coding sequence of the peptide that you want to be import enhanced. | ||
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| + | This part includes a ''HindIII'' restriction site as an extra. | ||
(refer to ''Characterisation of cell-penetrating peptide-mediated peptide delivery'' [Jones et al 2005] for more information about polyarginine) | (refer to ''Characterisation of cell-penetrating peptide-mediated peptide delivery'' [Jones et al 2005] for more information about polyarginine) | ||
Revision as of 03:09, 25 September 2012
Import enhancer - Polyarginine (trojan peptide)
Trojan peptides are short sequences that penetrate through the plasma membrane inside the cell without the need of any receptor or endocitosis process [Derossi 1998]. They can be used to increase the efficiency with which a protein enters a cell.
This part is Polyarginine, and has to be used just before the coding sequence of the peptide that you want to be import enhanced.
This part includes a HindIII restriction site as an extra.
(refer to Characterisation of cell-penetrating peptide-mediated peptide delivery [Jones et al 2005] for more information about polyarginine)
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]