Difference between revisions of "Part:BBa K864502:Design"

(→‎Design Notes)
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===Source===
 
===Source===
  
−
A BBa_J04450 RFP reporter, but modified by PCR mutagenesis.  
+
A <partinfo>BBa_J04450</partinfo> RFP reporter, but modified by PCR mutagenesis.
  
 
===References===
 
===References===

Revision as of 00:45, 25 September 2012

RFP Coding Device, constitutive


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 640
    Illegal AgeI site found at 752
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The CP44 promoter was chosen to be similar in strength to the LacI promoter, and to be used as a replacement for that one in the standard RFP cassette.

This part has not been assembled as a stand-alone BioBrick. It is a logical part of the pMB1ori-redq BBa_K864101, to be used as a default insert in low and middle copy backbones.

Source

A BBa_J04450 RFP reporter, but modified by PCR mutagenesis.

References