Difference between revisions of "Part:BBa K864502:Design"
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===Design Notes=== | ===Design Notes=== | ||
The CP44 promoter was chosen to be similar in strength to the LacI promoter, and to be used as a replacement for that one in the standard RFP cassette. | The CP44 promoter was chosen to be similar in strength to the LacI promoter, and to be used as a replacement for that one in the standard RFP cassette. | ||
+ | |||
+ | This part has not been assembled as a stand-alone BioBrick. It is a logical part of the pMB1ori-redq <partinfo>BBa_K864101</partinfo>, to be used as a default insert in low and middle copy backbones. | ||
===Source=== | ===Source=== |
Revision as of 00:41, 25 September 2012
RFP Coding Device, constitutive
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 640
Illegal AgeI site found at 752 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
The CP44 promoter was chosen to be similar in strength to the LacI promoter, and to be used as a replacement for that one in the standard RFP cassette.
This part has not been assembled as a stand-alone BioBrick. It is a logical part of the pMB1ori-redq BBa_K864101, to be used as a default insert in low and middle copy backbones.
Source
A BBa_J04450 RFP reporter, but modified by PCR mutagenesis.