Difference between revisions of "Part:BBa K925001"

 
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<partinfo>BBa_K925001 short</partinfo>
 
<partinfo>BBa_K925001 short</partinfo>
  
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This part is a delta-15 desaturase derived from Synechocystis sp PCC 6803.
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===Short description===
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The enzyme is able to introduce a double bond at the  &#916;-15 site in the hydrocarbon chain of linoleic acid (18:2 ; &#916;9,12), for its conversion into alpha- linoleic acid (18:3  ; &#916;9,12,15), an Omega-3 polyunsaturated fatty acid (PUFA).
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Delta-15 desaturase involved in an ω-3 biosynthetic pathway.
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Mass spectrometry results show that when feeding oleic acid to E. coli transformed with this part in addition to delta-12 desaturase (BBa_K925000), alpha-linoleic acid is observed in membrane lipids.  
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===Usage and Biology===
 
  
 
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===Description===
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===Functional Parameters===
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This part encodes a delta-15 desaturase derived from Synechocystis sp PCC 6803. The enzyme is able to introduce a double bond at the Δ-15 site in the hydrocarbon chain of linoleic acid (18:2 ; Δ9,12), to give alpha- linoleic acid (18:3 ; Δ9,12,15), an ω -3 polyunsaturated fatty acid (PUFA). Mass spectrometry results show that when feeding oleic acid to E. coli transformed with this part in addition to Δ -12 desaturase (BBa_K925000), alpha-linoleic acid is observed in membrane lipids.
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<partinfo>BBa_K925001 parameters</partinfo>
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===Characterisation===
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===Results===
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===Conclusion===

Revision as of 21:27, 24 September 2012

Delta 15 desaturase

Short description

Delta-15 desaturase involved in an ω-3 biosynthetic pathway.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Description

This part encodes a delta-15 desaturase derived from Synechocystis sp PCC 6803. The enzyme is able to introduce a double bond at the Δ-15 site in the hydrocarbon chain of linoleic acid (18:2 ; Δ9,12), to give alpha- linoleic acid (18:3 ; Δ9,12,15), an ω -3 polyunsaturated fatty acid (PUFA). Mass spectrometry results show that when feeding oleic acid to E. coli transformed with this part in addition to Δ -12 desaturase (BBa_K925000), alpha-linoleic acid is observed in membrane lipids.

Characterisation

Results

Conclusion