Difference between revisions of "Part:BBa K925000"
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<partinfo>BBa_K925000 short</partinfo> | <partinfo>BBa_K925000 short</partinfo> | ||
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<partinfo>BBa_K925000 SequenceAndFeatures</partinfo> | <partinfo>BBa_K925000 SequenceAndFeatures</partinfo> | ||
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+ | ===Description=== | ||
+ | This part encodes a delta-12 desaturase derived from Synechocystis sp PCC 6803. The enzyme is able to introduce a double bond at the Δ-12 site in the hydrocarbon chain of oleic acid (18:1, Δ9), for its conversion into linoleic acid (18:2 Δ9,12), a polyunsaturated fatty acid (PUFA). Mass spectrometry results show that when feeding oleic acid to E. coli transformed with this part, the bacterial membrane composition is changed, and linoleic acid is observed. | ||
===Functional Parameters=== | ===Functional Parameters=== | ||
<partinfo>BBa_K925000 parameters</partinfo> | <partinfo>BBa_K925000 parameters</partinfo> |
Revision as of 20:57, 24 September 2012
Delta-12 desaturase
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 426
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Description
This part encodes a delta-12 desaturase derived from Synechocystis sp PCC 6803. The enzyme is able to introduce a double bond at the Δ-12 site in the hydrocarbon chain of oleic acid (18:1, Δ9), for its conversion into linoleic acid (18:2 Δ9,12), a polyunsaturated fatty acid (PUFA). Mass spectrometry results show that when feeding oleic acid to E. coli transformed with this part, the bacterial membrane composition is changed, and linoleic acid is observed.