Difference between revisions of "Part:BBa K808030"

Revision as of 20:37, 24 September 2012

RBS-PhoA-His6tag-pNBEst13-Myctag-EstA

RBS-PhoA-His6tag-pNBEst13-Myctag-EstA is a long name for a long protein construct. It is a fusion protein which is secreted into the peri plasma in order to be integrated into the outer membran. When the phoA signal sequence is cut off, the N-terminus is formed by our pNB-Est13. This esterase shows actitivty towards PET and is anchored onto the cell by its inactive EstA membrane anchor.

Annotation: This site is about the construct and composition of our chimeric protein BBa_K808030. For any information about its expression rate or enzyme activity please visit BBa_K808032

Usage and Biology

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 1174
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 1700
Illegal NgoMIV site found at 1838
Illegal NgoMIV site found at 2378
Illegal NgoMIV site found at 2747
Illegal AgeI site found at 1245
1000
COMPATIBLE WITH RFC[1000]

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 __NOTOC__
 <partinfo>BBa_K808030 short</partinfo>
@@ Line 5: / Line 4: @@
 RBS-PhoA-His6tag-pNBEst13-Myctag-EstA is a long name for a long protein construct. It is a fusion protein which is secreted into the peri plasma in order to be integrated into the outer membran. When the phoA signal sequence is cut off, the N-terminus is formed by our pNB-Est13. This esterase shows actitivty towards PET and is anchored onto the cell by its inactive EstA membrane anchor.
-<!-- Add more about the biology of this part here
+Annotation: This site is about the construct and composition of our chimeric protein BBa_K808030. For any information about its expression rate or enzyme activity please visit [https://parts.igem.org/wiki/index.php?title=Part:BBa_K808032 BBa_K808032]
 ===Usage and Biology===