Difference between revisions of "Part:BBa K747001:Design"

 
(→‎Design Notes)
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<partinfo>BBa_K747001 short</partinfo>
 
<partinfo>BBa_K747001 short</partinfo>
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===Design Notes===
 
===Design Notes===
 
This protein domain can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg.  
 
This protein domain can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg.  
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The codon usage was modified to reduce homology in the sequence. A silent pointmutation was inserted into the BsmBI restriction site in the chloramphenicol gene
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The codon usage was modified to reduce homology in the sequence. A silent point mutation was inserted into the BsmBI restriction site in the chloramphenicol gene.
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===Source===
 
===Source===

Revision as of 17:55, 24 September 2012

TAL-Protein_AC1_Direpeat


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 64


Design Notes

This protein domain can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg. The codon usage was modified to reduce homology in the sequence. A silent point mutation was inserted into the BsmBI restriction site in the chloramphenicol gene.

Source

This Part was synthesized and flanked with BsmBI-restriction sites

References