Difference between revisions of "Part:BBa K875008:Experience"
| Line 2: | Line 2: | ||
This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | ||
how you used this part and how it worked out. | how you used this part and how it worked out. | ||
| − | + | ||
===Applications of BBa_K875008=== | ===Applications of BBa_K875008=== | ||
| + | '''Trieste 2012''' | ||
| + | |||
| + | [[Image:TsTse2021.jpg|center|500px]] | ||
| + | |||
| + | M15 bacteria cells (containing the pREP4 plasmid coding for the Lac repressor) are transformed with this Biobrick that resides on pSB1C3 plasmid. | ||
| + | |||
| + | After an over night liquid culture (LB broth) the OD600 absorbance is recorded and the culture is diluted to 0,1 and the batch is splitted into 6 flask – 20ml each. Three of these are induced with IPTG 1mM and the others are not induced. | ||
| + | |||
| + | The OD 600 is then recorded each hour. | ||
| + | It is possible to observe that not induced cells growth up to OD 1, instead the induced ones do not growth during the time, demonstrating the inducible promoter and toxin efficiency. | ||
===User Reviews=== | ===User Reviews=== | ||
<!-- DON'T DELETE --><partinfo>BBa_K875008 StartReviews</partinfo> | <!-- DON'T DELETE --><partinfo>BBa_K875008 StartReviews</partinfo> | ||
Revision as of 16:44, 24 September 2012
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K875008
Trieste 2012
M15 bacteria cells (containing the pREP4 plasmid coding for the Lac repressor) are transformed with this Biobrick that resides on pSB1C3 plasmid.
After an over night liquid culture (LB broth) the OD600 absorbance is recorded and the culture is diluted to 0,1 and the batch is splitted into 6 flask – 20ml each. Three of these are induced with IPTG 1mM and the others are not induced.
The OD 600 is then recorded each hour.
It is possible to observe that not induced cells growth up to OD 1, instead the induced ones do not growth during the time, demonstrating the inducible promoter and toxin efficiency.
User Reviews
UNIQ759c4c390f27eaf4-partinfo-00000000-QINU UNIQ759c4c390f27eaf4-partinfo-00000001-QINU