Difference between revisions of "Part:BBa K766004"

Revision as of 15:32, 24 September 2012

AndNot gate based on AHL

We design this A and not B gate. This part is based on the two kinds of AHL：C6HSL(signal A) and C12HSL(signal B). C6HSL can activate the promoter Plux and C12HSL can activate the promoter Plas. In this part, we add cI operator downstream of Plas. As a result, the promoter Plas can be activated by signal B but repressed by cI protein. The expression of cI protein is under the control of Plux, which can be activated by signal A. RFP is controled by Hybrid promoter(Plas and cI operator), which will work as a reporter.

In a word, the expression of RFP is only on the condition that A is absent and B is present.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 943
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 385
Illegal AgeI site found at 582
Illegal AgeI site found at 1630
Illegal AgeI site found at 1742
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 2859

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K766004 short</partinfo>
-We design this A and not B gate. This part is based on the two kinds of AHL&#65306;C6HSL(signal A) and C12HSL(signal B). C6HSL can activate the promoter Plux and C12HSL can activate the promoter Plas. In this part, we add cI operator downstream of Plas. As a result, the promoter Plas can ba activated by signal B but repressed by cI protein. The expression of cI protein is under the control of Plux, which can be activated by signal A. RFP is controled by Hybrid promoter(Plas and cI operator), which will work as a reporter.
+We design this A and not B gate. This part is based on the two kinds of AHL&#65306;C6HSL(signal A) and C12HSL(signal B). C6HSL can activate the promoter Plux and C12HSL can activate the promoter Plas. In this part, we add cI operator downstream of Plas. As a result, the promoter Plas can be activated by signal B but repressed by cI protein. The expression of cI protein is under the control of Plux, which can be activated by signal A. RFP is controled by Hybrid promoter(Plas and cI operator), which will work as a reporter.
 In a word, the expression of RFP is only on the condition that A is absent and B is present.