Difference between revisions of "Part:BBa K801020"

Revision as of 14:51, 24 September 2012

KlADH4 yeast promoter, ethanol inducible

This part is the ethanol inducible promoter controlling the KlADH4-gene of K. lactis.

The use of this ethanol inducible promoter to produce heterologous proteins in K. lactis was shown by Salioa et al. 1999 [http://www.ncbi.nlm.nih.gov/pubmed?term=9872759].

We characterized this part in S. cerevisiae (strain INVSc1) to find out whether this part is also ethanol-inducible in this yeast.

Usage and Biology

The UASe-region of this promoter has been shown to be responsible for the ethanol sensitivity of this promoter in K. lactis (Mazzoni et al., 2000 [http://www.ncbi.nlm.nih.gov/pubmed?term=10724480]). The region includes binding sites for the Rap1-protein (repressor activator protein 1) and the Yap1-protein (a transcription factor involved in stress response) as well as two heat shock elements (HSE) and five stress response elemtents (STRE). All these cis-elements and the respecitve proteins also occur in S. cerevisiae. For this reason we wanted to examine whether the KlADH4-promoter remains ethanol inducible if it is transferred from its natural organism (K. lactis) to S. cerevisiae.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 753
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 590

References

[1] Saliola, M., Mazzoni, C., Solimando, N., Crisà, A., Falcone, C. & Jung, G. (1999) ‚Use of the KlADH4 promoter for ethanol-dependent production of recombinant human serum albumin in Kluyveromyces lactis’, Appl Environ Microbiol. 65 (1), 53-60. [http://www.ncbi.nlm.nih.gov/pubmed?term=10724480 PMID: 9872759]

[2] Mazzoni, C., Santori, F., Saliola, M. & Falcone, C. (2000) ‚Molecular analysis of UASE, a cis element containing stress response elements responsible for ethanol induction of the KlADH4 gene of Kluyveromyces lactis’, Res. Microbiol. 151, 19-28. [http://www.ncbi.nlm.nih.gov/pubmed?term=10724480 PMID: 10724480]

@@ Line 9: / Line 9: @@
-<!-- Add more about the biology of this part here
 ===Usage and Biology===
-<!-- -->
+The UASe-region of this promoter has been shown to be responsible for the ethanol sensitivity of this promoter in ''K. lactis (Mazzoni et al., 2000 [http://www.ncbi.nlm.nih.gov/pubmed?term=10724480]). The region includes binding sites for the Rap1-protein (repressor activator protein 1) and the Yap1-protein (a transcription factor involved in stress response) as well as two heat shock elements (HSE) and five stress response elemtents (STRE). All these cis-elements and the respecitve proteins also occur in ''S. cerevisiae''. For this reason we wanted to examine whether the KlADH4-promoter remains ethanol inducible if it is transferred from its natural organism (''K. lactis'') to ''S. cerevisiae''.
 <span class='h3bb'>Sequence and Features</span>
 <partinfo>BBa_K801020 SequenceAndFeatures</partinfo>
@@ Line 24: / Line 24: @@
 ===References===
-* [1] Saliola, M., Mazzoni, C., Solimando, N., Crisà, A., Falcone, C. & Jung, G. (1999) ‚Use of the
+* [1] Saliola, M., Mazzoni, C., Solimando, N., Crisà, A., Falcone, C. & Jung, G. (1999) ''‚Use of the KlADH4 promoter for ethanol-dependent production of recombinant human serum albumin in Kluyveromyces lactis’'', '''Appl Environ Microbiol.''' 65 (1), 53-60. [http://www.ncbi.nlm.nih.gov/pubmed?term=10724480 PMID: 9872759]
-KlADH4 promoter for ethanol-dependent production of recombinant human serum albumin
-in Kluyveromyces lactis’, Appl Environ Microbiol. 65 (1), 53-60.
+* [2] Mazzoni, C., Santori, F., Saliola, M. & Falcone, C. (2000) ''‚Molecular analysis of UASE, a cis element containing stress response elements responsible for ethanol induction of the KlADH4 gene of Kluyveromyces lactis’,''  '''Res. Microbiol.''' 151, 19-28. [http://www.ncbi.nlm.nih.gov/pubmed?term=10724480 PMID: 10724480]