Difference between revisions of "Part:BBa K801020"

 
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<partinfo>BBa_K801020 short</partinfo>
 
<partinfo>BBa_K801020 short</partinfo>
  
 
This part is the ethanol inducible promoter controlling the KlADH4-gene of ''K. lactis''.  
 
This part is the ethanol inducible promoter controlling the KlADH4-gene of ''K. lactis''.  
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The use of this ethanol inducible promoter to produce heterologous proteins in ''K. lactis'' was shown by Salioa et al. 1999 [http://www.ncbi.nlm.nih.gov/pubmed?term=9872759].
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We characterized this part in ''S. cerevisiae'' (strain INVSc1) to find out whether this part is also ethanol-inducible in this yeast.
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<partinfo>BBa_K801020 parameters</partinfo>
 
<partinfo>BBa_K801020 parameters</partinfo>
 
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===References===
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* [1] Saliola, M., Mazzoni, C., Solimando, N., Crisà, A., Falcone, C. & Jung, G. (1999) ‚Use of the
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KlADH4 promoter for ethanol-dependent production of recombinant human serum albumin
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in Kluyveromyces lactis’, Appl Environ Microbiol. 65 (1), 53-60.

Revision as of 14:39, 24 September 2012

KlADH4 yeast promoter, ethanol inducible

This part is the ethanol inducible promoter controlling the KlADH4-gene of K. lactis.

The use of this ethanol inducible promoter to produce heterologous proteins in K. lactis was shown by Salioa et al. 1999 [http://www.ncbi.nlm.nih.gov/pubmed?term=9872759].

We characterized this part in S. cerevisiae (strain INVSc1) to find out whether this part is also ethanol-inducible in this yeast.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 753
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 590



References

  • [1] Saliola, M., Mazzoni, C., Solimando, N., Crisà, A., Falcone, C. & Jung, G. (1999) ‚Use of the

KlADH4 promoter for ethanol-dependent production of recombinant human serum albumin in Kluyveromyces lactis’, Appl Environ Microbiol. 65 (1), 53-60.