Difference between revisions of "Part:BBa K929004"

Revision as of 14:36, 24 September 2012

modified AID+eGFP

This is a composite part from the existing parts: modified_AID (BBa_929001) and eGFP (BBa_K404316). Modified_AID (BBa_929001) is in RFC 10 cloning standard and has an aditional AgeI restriciton site. Therefore it can be fused with RFC 25 parts (C-terminal of modified AID).This part was not used to express the fusion protein. It is an usefull intermediate that can be combined with differnt promoters and terminators. We combined this part with CMV-promoter and polyA-hGH (poly adenylation signal sequence)--> see BBa_929002.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 1296
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 108
Illegal BsaI.rc site found at 1225
Illegal SapI site found at 209

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K929004 short</partinfo>
-This is a composite part from the existing parts: modified_AID (BBa_929001) and eGFP (BBa_K404316). We used RFC 25 enzymes to create this part as a fusion of modified AID and eGFP (at the C-terminus of mod_AID). This part was '''not''' used to express the fusion protein. It is an usefull intermediate that can be combined with differnt promoters and terminators. We combined this part with CMV-promoter and polyA-hGH (poly adenylation signal sequence)--> see BBa_929002.
+This is a composite part from the existing parts: modified_AID (BBa_929001) and eGFP (BBa_K404316). Modified_AID (BBa_929001) is in RFC 10 cloning standard and has an aditional AgeI restriciton site. Therefore it can be fused with RFC 25 parts (C-terminal of modified AID).This part was '''not''' used to express the fusion protein. It is an usefull intermediate that can be combined with differnt promoters and terminators. We combined this part with CMV-promoter and polyA-hGH (poly adenylation signal sequence)--> see BBa_929002.
 <!-- Add more about the biology of this part here