Difference between revisions of "Part:BBa K801020:Design"
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===Design Notes=== | ===Design Notes=== | ||
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| + | To remove a naturally occuring SpeI-restriction site, the naturally occuring "A" at position 757 was changed to "T" using quickchange mutagenesis. | ||
| + | The feature annotation in the UASe region (Upstream activating sequence "ethanol") is taken from: | ||
| + | * Mazzoni, C., Santori, F., Saliola, M. & Falcone, C. (2000) ‚Molecular analysis of UASE, a cis element containing stress response elements responsible for ethanol induction of the KlADH4 gene of Kluyveromyces lactis’, Res. Microbiol. 151, 19-28. PMID: 10724480 | ||
===Source=== | ===Source=== | ||
Revision as of 14:32, 24 September 2012
KlADH4 yeast promoter, ethanol inducible
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 753
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 590
Design Notes
To remove a naturally occuring SpeI-restriction site, the naturally occuring "A" at position 757 was changed to "T" using quickchange mutagenesis.
The feature annotation in the UASe region (Upstream activating sequence "ethanol") is taken from:
- Mazzoni, C., Santori, F., Saliola, M. & Falcone, C. (2000) ‚Molecular analysis of UASE, a cis element containing stress response elements responsible for ethanol induction of the KlADH4 gene of Kluyveromyces lactis’, Res. Microbiol. 151, 19-28. PMID: 10724480
Source
Extracted from genomic DNA of K. lactis using PCR.