Difference between revisions of "Part:BBa K733005:Design"

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===Source===
 
===Source===
  
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Extract BMP-2 from mouse genomic DNA by PCR. Extract signal peptide YbdN from ''Bacillus subtilis'' genomic DNA by PCR. Ligation of YbdN and BMP2 achieved by overlapping PCR.
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''BMP-2'' gene is obtained from mouse genomic DNA by PCR. Signal peptide ''YbdN'' gene is obtained from ''Bacillus subtilis'' genomic DNA by PCR. The recombinant protein ''YbdN+BMP-2'' is obtained by overlapping PCR.
  
 
===References===
 
===References===

Revision as of 13:24, 24 September 2012

ybdN+Bmp2


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The mouse BMP-2 DNA sequence has an EcoRI cutting site and this site is within the codon of the DNA sequence. To standardize the biobrick, we try to do a point mutation on mouse BMP-2 after we amplify it from PCR. The purpose is to remove the EcoRI cutting site and remain the correct codon for protein translation of BMP-2.

Source

BMP-2 gene is obtained from mouse genomic DNA by PCR. Signal peptide YbdN gene is obtained from Bacillus subtilis genomic DNA by PCR. The recombinant protein YbdN+BMP-2 is obtained by overlapping PCR.

References