Difference between revisions of "Part:BBa K801077"

Revision as of 12:33, 24 September 2012

Caffeine Synthesis Pathway pTEF2-XMT1-tADH1-pTEF1-MXMT1-tADH1-pTEF2-DXMT1-tADH1

This part allows the expression of the three enzymes CaXMT1, CaMXMT1 and CaDXMT1 in yeast. These enzymes form the biosynthesis pathway for caffeine.

The expression of the enzymes CaXMT1 (Coffea arabica xanthosine-N-methyl-transferase 1, BBa_K801070) and CaDXMT1 (Coffea arabica dimethyl-xanthine N-methyl transferase 1, = caffeine synthase, BBa_K801072) is controlled by the TEF2-promoter (BBa_K801010) and the ADH1-terminator (BBa_K801012)

The expression of CaMXMT1 (Coffea arabica methyl-xanthine N-methyl transferase 1, BBa_K801071) is controlled by the TEF1 promoter (BBa_K319003) and the ADH1-terminator (BBa_K801012)

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 3613
Illegal BglII site found at 5829
Illegal BglII site found at 5925
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 138
Illegal NgoMIV site found at 4317
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 2385

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K801077 short</partinfo>
 This part allows the expression of the three enzymes CaXMT1, CaMXMT1 and CaDXMT1 in '''yeast'''. These enzymes form the biosynthesis pathway for caffeine.
 The expression of the enzymes CaXMT1 (''Coffea arabica'' xanthosine-N-methyl-transferase 1, BBa_K801070) and CaDXMT1 (''Coffea arabica'' dimethyl-xanthine N-methyl transferase 1, = caffeine synthase, BBa_K801072) is controlled by the TEF2-promoter (BBa_K801010) and the ADH1-terminator (BBa_K801012)
 The expression of CaMXMT1 (''Coffea arabica'' methyl-xanthine N-methyl transferase 1, BBa_K801071) is controlled by the TEF1 promoter (BBa_K319003) and the ADH1-terminator (BBa_K801012)