Difference between revisions of "Part:BBa K929106"

 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K929106 short</partinfo>
 
<partinfo>BBa_K929106 short</partinfo>
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{| style="color:black" cellpadding="6" cellspacing="1" border="2" align="right"
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! colspan="2" style="background:#66bbff;"|[https://parts.igem.org/Part:BBa_K929003 B-cell transmembrane region with mCherry and TEV site, framed by LoxP]
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|-
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! colspan="2"|[[Image:|300px]]
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|-
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|'''BioBrick Nr.'''
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|[https://parts.igem.org/Part:BBa_929103 BBa_929103]
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|-
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|'''RFC standard'''
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|[https://parts.igem.org/Help:Assembly_standard_25 RFC 25]
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|-
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|'''Requirement'''
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|pSB1C3<br>
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|-
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|'''Source'''
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|Gene synthesis by GeneArt
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|-
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|'''Submitted by'''
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|[http://2012.igem.org/Team:Potsdam_Bioware Potsdam_Bioware2012]
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|}
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<br>
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[[Image:.png|left|thumb|400px]]<br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br>
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<br>
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<br>
 
This part is a composite part of the transmembrane domain of a B-cell receptor with a 3 aa linker fused to the mcherry reporter.
 
This part is a composite part of the transmembrane domain of a B-cell receptor with a 3 aa linker fused to the mcherry reporter.
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<br>
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<br>
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Transmembrane domain of B-cell receptor with glycine-serine linker:<br>
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Helical single-span transmembrane domain of the B-Cell-Receptor with a flexible 3 aa linker at the C-terminal end. This part was designed for fusion to proteins or peptides that will be presented on the cells surface. A signal peptide at the N-terminal end of the fusion protein for membrane integration (e.g. part BBa_K157001) is highly required.
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<br>
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<br>
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mCherry:<br>
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Expression of the fusion part and its cellular localization can be monitored by the mcherry signal with an excitation at 587 nm and an emission at 610 nm.
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<br>
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<br>
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===

Revision as of 11:35, 24 September 2012

B-cell transmembrane region with mCherry

B-cell transmembrane region with mCherry and TEV site, framed by LoxP
[[Image:|300px]]
BioBrick Nr. BBa_929103
RFC standard RFC 25
Requirement pSB1C3
Source Gene synthesis by GeneArt
Submitted by [http://2012.igem.org/Team:Potsdam_Bioware Potsdam_Bioware2012]


File:.png
400px





















This part is a composite part of the transmembrane domain of a B-cell receptor with a 3 aa linker fused to the mcherry reporter.

Transmembrane domain of B-cell receptor with glycine-serine linker:
Helical single-span transmembrane domain of the B-Cell-Receptor with a flexible 3 aa linker at the C-terminal end. This part was designed for fusion to proteins or peptides that will be presented on the cells surface. A signal peptide at the N-terminal end of the fusion protein for membrane integration (e.g. part BBa_K157001) is highly required.

mCherry:
Expression of the fusion part and its cellular localization can be monitored by the mcherry signal with an excitation at 587 nm and an emission at 610 nm.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]