Difference between revisions of "Part:BBa K883151"

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===Usage and Biology===
 
===Usage and Biology===
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==Usage==
  
 
CCMV is an abriviation of the Cowpea Chlorotic Mottle Virus, one of the most studied viruses in producing Virus Like Particles (VLPs). This part is the produces a coat protein of the Cowpea Clorotic Mottle Virus (CCMV), with the first 25 amino acids replaced with an Histidine tag (6x).When expressed in ''Escherichia coli'', these coat proteins can be harvested and be assembled in vitro using the Wageningen_UR iGEM 2012 protocols.
 
CCMV is an abriviation of the Cowpea Chlorotic Mottle Virus, one of the most studied viruses in producing Virus Like Particles (VLPs). This part is the produces a coat protein of the Cowpea Clorotic Mottle Virus (CCMV), with the first 25 amino acids replaced with an Histidine tag (6x).When expressed in ''Escherichia coli'', these coat proteins can be harvested and be assembled in vitro using the Wageningen_UR iGEM 2012 protocols.
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<li>[http://2012.igem.org/Team:Wageningen_UR/Protocol/RoundupCCMV| CCMV purification protocol]</li>
 
<li>[http://2012.igem.org/Team:Wageningen_UR/Protocol/RoundupCCMV| CCMV purification protocol]</li>
 
</ul>
 
</ul>
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==Biology==
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Revision as of 10:52, 24 September 2012

CCMV coat protein gene 25AA N-terminal replaced with HIStag under IPTG inducable promotor

This part produces a coat protein of the Cowpea Clorotic Mottle Virus (CCMV), with the first 25 amino acids replaced with an Histidine tag (6x).

Usage and Biology

Usage

CCMV is an abriviation of the Cowpea Chlorotic Mottle Virus, one of the most studied viruses in producing Virus Like Particles (VLPs). This part is the produces a coat protein of the Cowpea Clorotic Mottle Virus (CCMV), with the first 25 amino acids replaced with an Histidine tag (6x).When expressed in Escherichia coli, these coat proteins can be harvested and be assembled in vitro using the Wageningen_UR iGEM 2012 protocols.


Protocols for production and purification:

  • [http://2012.igem.org/Team:Wageningen_UR/Protocol/StartupCCMV| CCMV production protocol part 1]
  • [http://2012.igem.org/Team:Wageningen_UR/Protocol/DialysisCCMV| CCMV production protocol part 2]
  • [http://2012.igem.org/Team:Wageningen_UR/Protocol/RoundupCCMV| CCMV purification protocol]

Biology

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Functional Parameters

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