Difference between revisions of "Part:BBa K929103:Design"

 
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===References===
 
===References===
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[1] Araki K, Imaizumi T, Okuyama K, Oike Y, Yamamura KI (1997) Efficiency of recombination by Cre transient expression in embryonic stem cells: comparison of various promoters. J Biochem; 122, 977-82

Revision as of 10:36, 24 September 2012

B-cell transmembrane region with mCherry and TEV site, framed by LoxP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Gene synthesis by GeneArt, optimized for expression in CHO cells (Cricetulus griseus).


Source

TEV: TEV Protease recognition site according to life technologies manual (AcTEV TM Protease),gene synthesis by GeneArt, optimized for expression in CHO cells (Cricetulus griseus)

LoxP sites: two for Cre mediated recombination, gene synthesis by GeneArt, optimized for expression in CHO cells (Cricetulus griseus)

Transmembrane domain: transmembrane domain of B-cell receptor with glycine-serine linker (BBa_K157010),gene synthesis by GeneArt, optimized for expression in CHO cells (Cricetulus griseus)

Reportergene: mcherry, gene synthesis by GeneArt, optimized for expression in CHO cells (Cricetulus griseus)


References

[1] Araki K, Imaizumi T, Okuyama K, Oike Y, Yamamura KI (1997) Efficiency of recombination by Cre transient expression in embryonic stem cells: comparison of various promoters. J Biochem; 122, 977-82