Difference between revisions of "Part:BBa K897318"
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<partinfo>BBa_K897318 short</partinfo> | <partinfo>BBa_K897318 short</partinfo> | ||
| − | As we know, all antisense RNAs had a short half-life of about 1 minute. Because antisense RNA efficiency is determined by the binding rate to the target, which in turn is determined by both antisense RNA concentration and binding rate constant, a higher | + | As we know, all antisense RNAs had a short half-life of about 1 minute. Because antisense RNA efficiency is determined by the binding rate to the target, |
| + | which in turn is determined by both antisense RNA concentration and binding rate constant, a higher | ||
intracellular concentration of the inhibitor will result in higher efficacy. | intracellular concentration of the inhibitor will result in higher efficacy. | ||
Revision as of 04:58, 24 September 2012
Paired termini structure
As we know, all antisense RNAs had a short half-life of about 1 minute. Because antisense RNA efficiency is determined by the binding rate to the target, which in turn is determined by both antisense RNA concentration and binding rate constant, a higher
intracellular concentration of the inhibitor will result in higher efficacy.
An alternative strategy to stabilize asRNAs is to pair the termini using flanking inverted repeats to create a hairpin structure with the antisense sequence within a large loop. A paired termini (PT) design, where flanking inverted repeats create paired asRNA
termini,was proved can produce effective gene silencing. PT asRNAs are abundant and stable and function through an RNase III independent mechanism that requires a large stoichiometric excess of asRNA.PT consists of non-endogenous GC-rich sequences and has a stem-loop structure, which can improve the stability of as RNA raises its abundance.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 59
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 59
Illegal NotI site found at 84 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 59
Illegal BamHI site found at 53
Illegal XhoI site found at 93 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 59
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 59
- 1000COMPATIBLE WITH RFC[1000]