Difference between revisions of "Part:BBa K743018"

Revision as of 00:55, 24 September 2012

sfGFP.degradation.tag under Pta promoter in psb1C3_IntKR plasmid

This is a circadian clock controlled reporter for Synechocystis PCC6803. Placed in the psb1C3_IntKR integrative plasmid it has sfGFP with an N-terminal fast degradation tag under the control of the Pta promoter wich comes from a Synechocystis gene known to oscillate in a circadian manner.

The short live of this tagged sfGFP allows for the caracterization of the activity of any promoter, in this case, the hypothesized oscillation of the Pta promoter.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal EcoRI site found at 2699
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 2699
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 2699
Illegal XhoI site found at 3196
23
INCOMPATIBLE WITH RFC[23]
Illegal EcoRI site found at 2699
25
INCOMPATIBLE WITH RFC[25]
Illegal EcoRI site found at 2699
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI site found at 511
Illegal SapI.rc site found at 699

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 __NOTOC__
 <partinfo>BBa_K743018 short</partinfo>
+This is a circadian clock controlled reporter for Synechocystis PCC6803. Placed in the [https://parts.igem.org/Part:BBa_K743008 psb1C3_IntKR integrative plasmid] it has sfGFP with an N-terminal fast degradation tag under the control of the [https://parts.igem.org/Part:BBa_K743003 Pta promoter] wich comes from a Synechocystis gene known to oscillate in a circadian manner.
+The short live of this tagged sfGFP allows for the caracterization of the activity of any promoter, in this case, the hypothesized oscillation of the Pta promoter.