Difference between revisions of "Part:BBa K823019"
Line 4: Line 4:
 
This lacZ gene is derived from the Bacillus reporter vector pAC6. It is constructed in the [[Help:Assembly_standard_25|Freiburg standard]] with RBS.
 
This lacZ gene is derived from the Bacillus reporter vector pAC6. It is constructed in the [[Help:Assembly_standard_25|Freiburg standard]] with RBS.
  
−
 
+
lacZ is a β-galactosidase which cleaves the substrates X-Gal (5-bromo-4-chloro-indolyl-β-D-galactopyranoside) and ONPG (''ortho''-Nitrophenyl-β-galactoside).
  
 
prefix: GAATTCCGCGGCCGCTTCTAGATAAGGAGGAACTACTATGGCCGGC
 
prefix: GAATTCCGCGGCCGCTTCTAGATAAGGAGGAACTACTATGGCCGGC
  
 
suffix: ACCGGTTAATACTAGTAGCGGCCGCTGCAGT
 
suffix: ACCGGTTAATACTAGTAGCGGCCGCTGCAGT
−
 
−
 
  
  

Revision as of 19:12, 23 September 2012

LacZ (RBS+Freiburg standard)

This lacZ gene is derived from the Bacillus reporter vector pAC6. It is constructed in the Freiburg standard with RBS.

lacZ is a β-galactosidase which cleaves the substrates X-Gal (5-bromo-4-chloro-indolyl-β-D-galactopyranoside) and ONPG (ortho-Nitrophenyl-β-galactoside).

prefix: GAATTCCGCGGCCGCTTCTAGATAAGGAGGAACTACTATGGCCGGC

suffix: ACCGGTTAATACTAGTAGCGGCCGCTGCAGT


We used this lacZ gene to evaluate our promoters.

lacZ under the control of Pspac in pSB1C3. Plate was induced with xylose.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]