Difference between revisions of "Part:BBa K823052"

Revision as of 19:12, 23 September 2012

lacZ, B. subtilis optimized + RBS + Term

lacZ in Freiburg standard, Bacillus subtilis codon optimized with ribosome binding site and terminator (B0014).

lacZ is a β-galactosidase which cleaves the substrates X-Gal (5-bromo-4-chloro-indolyl-β-D-galactopyranoside) and ONPG (ortho-Nitrophenyl-β-galactoside).

This BioBrick was used for promoter evaluation in B. subtilis.

prefix: GAATTCCGCGGCCGCTTCTAGATAAGGAGGAACTACTATGGCCGGC

suffix: ACCGGTTAATACTAGTAGCGGCCGCTGCAGT

We used this lacZ gene to evaluate our promoters.

lacZ under the control of Pspac in pSB1C3. Plate was induced with xylose.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K823052 short</partinfo>
-lacZ in Freiburg standard, ''Bacillus subtilis'' codon optimized with ribosome binding site.
+lacZ in Freiburg standard, ''Bacillus subtilis'' codon optimized with ribosome binding site and terminator (B0014).
 lacZ is a β-galactosidase which cleaves the substrates X-Gal (5-bromo-4-chloro-indolyl-β-D-galactopyranoside) and ONPG (''ortho''-Nitrophenyl-β-galactoside).
 This BioBrick was used for promoter evaluation in ''B. subtilis''.
+prefix: GAATTCCGCGGCCGCTTCTAGATAAGGAGGAACTACTATGGCCGGC
+suffix: ACCGGTTAATACTAGTAGCGGCCGCTGCAGT
+We used this ''lacZ'' gene to evaluate our promoters.
+[[Image:LacZ plate.png|thumb|center|400px|lacZ under the control of Pspac in pSB1C3. Plate was induced with xylose.]]
 <!-- Add more about the biology of this part here