Difference between revisions of "Part:BBa K823052"

 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K823052 short</partinfo>
 
<partinfo>BBa_K823052 short</partinfo>
  
lacZ in Freiburg standard, B. subtilis codon optimized + RBS + Term (B0014)
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lacZ in Freiburg standard, ''Bacillus subtilis'' codon optimized with ribosome binding site.
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lacZ is a β-galactosidase which cleaves the substrates X-Gal (5-bromo-4-chloro-indolyl-β-D-galactopyranoside) and ONPG (''ortho''-Nitrophenyl-β-galactoside).
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This BioBrick was used for promoter evaluation in ''B. subtilis''.
  
 
<!-- Add more about the biology of this part here
 
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Revision as of 19:10, 23 September 2012

lacZ, B. subtilis optimized + RBS + Term

lacZ in Freiburg standard, Bacillus subtilis codon optimized with ribosome binding site.

lacZ is a β-galactosidase which cleaves the substrates X-Gal (5-bromo-4-chloro-indolyl-β-D-galactopyranoside) and ONPG (ortho-Nitrophenyl-β-galactoside).

This BioBrick was used for promoter evaluation in B. subtilis.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]