Difference between revisions of "Part:BBa K823052"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K823052 short</partinfo> | <partinfo>BBa_K823052 short</partinfo> | ||
− | lacZ in Freiburg standard, | + | lacZ in Freiburg standard, ''Bacillus subtilis'' codon optimized with ribosome binding site. |
+ | |||
+ | lacZ is a β-galactosidase which cleaves the substrates X-Gal (5-bromo-4-chloro-indolyl-β-D-galactopyranoside) and ONPG (''ortho''-Nitrophenyl-β-galactoside). | ||
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+ | This BioBrick was used for promoter evaluation in ''B. subtilis''. | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here |
Revision as of 19:10, 23 September 2012
lacZ, B. subtilis optimized + RBS + Term
lacZ in Freiburg standard, Bacillus subtilis codon optimized with ribosome binding site.
lacZ is a β-galactosidase which cleaves the substrates X-Gal (5-bromo-4-chloro-indolyl-β-D-galactopyranoside) and ONPG (ortho-Nitrophenyl-β-galactoside).
This BioBrick was used for promoter evaluation in B. subtilis.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]