Difference between revisions of "Part:BBa K823024"
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<partinfo>BBa_K823024 short</partinfo>
 
<partinfo>BBa_K823024 short</partinfo>
  
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''This part is an expression vector for Bacillus subtilis. It integrates at the thrC locus. It has a ampicillin resistance for cloning in E.coli and chloramphenicol resistance for selection in B.subtilis. The multiple cloning site downstream of a Pxyl promoter, that is inducible with xylose.
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</>
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This part is an expression vector for Bacillus subtilis. It integrates at the thrC locus. It has a ampicillin resistance for cloning in E.coli and chloramphenicol resistance for selection in B.subtilis. The multiple cloning site downstream of a Pxyl promoter, that is inducible with xylose.
 
This backbone is a ''BioBricked'' version of the ''B. subtilis'' vector pXT.
 
This backbone is a ''BioBricked'' version of the ''B. subtilis'' vector pXT.
  
 
[[Image:LMU-Munich-PSBBs4S-Pxyl.png|600px]]
 
[[Image:LMU-Munich-PSBBs4S-Pxyl.png|600px]]
  
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CAUTION: The submitted BioBrick can somehow not be transformed into ''Bacillus subtilis''. Please do not use this part. When we fixed the problem, we will submit a working version or the detailed protocol!
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==CAUTION: The submitted BioBrick can somehow not be transformed into ''Bacillus subtilis''. Please do not use this part. When we fixed the problem, we will submit a working version or the detailed protocol!==
  
 
This BioBrick is part of the LMU 2012 igem project [http://2012.igem.org/Team:LMU-Munich/Bacillus_BioBricks '''''Bacillus''B'''io'''B'''rick'''B'''ox]
 
This BioBrick is part of the LMU 2012 igem project [http://2012.igem.org/Team:LMU-Munich/Bacillus_BioBricks '''''Bacillus''B'''io'''B'''rick'''B'''ox]

Revision as of 17:48, 23 September 2012

pSBBs4S-Pxyl: Integrative expression vector for Bacillus subtilis


</> This part is an expression vector for Bacillus subtilis. It integrates at the thrC locus. It has a ampicillin resistance for cloning in E.coli and chloramphenicol resistance for selection in B.subtilis. The multiple cloning site downstream of a Pxyl promoter, that is inducible with xylose. This backbone is a BioBricked version of the B. subtilis vector pXT.

LMU-Munich-PSBBs4S-Pxyl.png

CAUTION: The submitted BioBrick can somehow not be transformed into Bacillus subtilis. Please do not use this part. When we fixed the problem, we will submit a working version or the detailed protocol!

This BioBrick is part of the LMU 2012 igem project [http://2012.igem.org/Team:LMU-Munich/Bacillus_BioBricks BacillusBioBrickBox]

Reference: [http://www.ncbi.nlm.nih.gov/pubmed/11069659 Derré et al.]



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 4773
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 4779
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 4773
    Illegal BamHI site found at 4757
    Illegal XhoI site found at 1011
    Illegal XhoI site found at 1929
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 4773
    Illegal suffix found at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 4773
    Plasmid lacks a suffix.
    Illegal XbaI site found at 4788
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NgoMIV site found at 1893
    Illegal NgoMIV site found at 3032
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI.rc site found at 2856
    Illegal SapI.rc site found at 605