Difference between revisions of "Part:BBa K784000:Experience"
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We have used the part inside a BL21 strain of ''E.coli'' which has an endogenouse PET system. <br> | We have used the part inside a BL21 strain of ''E.coli'' which has an endogenouse PET system. <br> | ||
In the presence of IPTG inducer, T7 RNAP is expressed and activates the part promoter which expresses the Alkaline Phosphatase gene (phoA).<br> | In the presence of IPTG inducer, T7 RNAP is expressed and activates the part promoter which expresses the Alkaline Phosphatase gene (phoA).<br> | ||
| − | We checked the absorbance resulted from the [https://parts.igem.org/Arkin_JCA_PNPAssay PNP (para-nitrophenol phosphate) hydrolysis assay] in raising concetrations of the inducer (IPTG) | + | We checked the absorbance resulted from the [https://parts.igem.org/Arkin_JCA_PNPAssay PNP (para-nitrophenol phosphate) hydrolysis assay] in raising concetrations of the inducer (IPTG).The error bars represent the processing of the data collected. The line at the bottom of the graph represents the basal level according to the control result- no IPTG induction.The results are before you in the graph: <br> |
[[Image:technionphoagraph.jpg|400X400px|frame|center]] | [[Image:technionphoagraph.jpg|400X400px|frame|center]] | ||
Revision as of 15:36, 23 September 2012
This experience page is provided so that any user may enter their experience using this part.
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how you used this part and how it worked out.
Applications of BBa_K784000
We have used the part inside a BL21 strain of E.coli which has an endogenouse PET system.
In the presence of IPTG inducer, T7 RNAP is expressed and activates the part promoter which expresses the Alkaline Phosphatase gene (phoA).
We checked the absorbance resulted from the PNP (para-nitrophenol phosphate) hydrolysis assay in raising concetrations of the inducer (IPTG).The error bars represent the processing of the data collected. The line at the bottom of the graph represents the basal level according to the control result- no IPTG induction.The results are before you in the graph:
As you can see in the graph, raising concentrations of the inducer results in raising absorbance, which means raising concentration of the Alkaline Phosphatase enzyme.
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UNIQcc52c371a7c5b1f7-partinfo-00000000-QINU UNIQcc52c371a7c5b1f7-partinfo-00000001-QINU